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Bis-ANS is superior to 1-8-ANS as a probe for nonpolar cavities in proteins, often binding with an affinity that is orders-of-magnitude higher. Anilinonaphthalene sulfonates Invitrogen Bis-ANS (4,4'-Dianilino-1,1'-Binaphthyl-5,5'-Disulfonic Acid, | Fisher Scientific


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Background and objective: Published algorithms for classification of human sperm heads are based on relatively small image databases that are not open to the public, and thus no direct comparison is available for competing methods. We describe a gold-standard for morphological sperm analysis (SCIAN-MorphoSpermGS), a dataset of sperm head images with expert-classification labels in one of the.


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Bis-ANS is a fluorescent probe for nonpolar cavities in proteins. Used to detect A β fibre (K d = ~80 nM); exhibits distinct fluorescent profiles for fibres and oligomers. Fluoresces in hydrophobic environments, negligible fluorescence in water solutions. Excitation\emission λ ~ 355\520 nm at pH 7.4.


(A) Change of bisANS fluorescence intensity (fluorescence maximum, λ

These results resemble effects of bis-ANS on BSA, where low ratios of bis-ANS:protein were found to increase thermostability of BSA, while higher ratios induced transition of the protein into a molten globule-like state (Celej et al. 2003). Therefore, we propose that accelerated aggregation of IL-1ra in the presence of 4.2-21 mM ANS reflects.


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compound Summary 4,4'-Dianilino-1,1'-binaphthyl-5,5'-disulfonic acid dipotassium salt PubChem CID 16213473 Structure Chemical Safety Laboratory Chemical Safety Summary (LCSS) Datasheet Molecular Formula C32H22K2N2O6S2 Synonyms Bis-ans 65664-81-5 4,4'-Dianilino-1,1'-binaphthyl-5,5'-disulfonic acid dipotassium salt Bis-ANS (dipotassium)


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Bis-ANS was the most effective inhibitor of prion peptide aggregation. Bis-ANS binds strongly to rPrP 23-231 leading to a substantial increase in -sheet content and to limited oligomerization.


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bis-ANS is a high-affinity non-covalent extrinsic fluorescent dye used to analyze protein conformation.1 Its predominant interaction with proteins is through its hydrophobic phenyl and naphthyl rings.2 bis-ANS has an excitation maxima of 390 nm.3 It has an emission maximum of 523 nm when free in solution but


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ANS has the potential to characterize proteins in solid states. • ANS fluorescence in protein films reveals the hydrophobic sites with distinct properties. • Short lifetime DAS of ANS in hydrated protein films is similar to that of ANS-protein complexes in solution. •


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Product Overview Documents Bis-ANS is superior to 1-8-ANS as a probe for nonpolar cavities in proteins, often binding with an affinity that is orders-of-magnitude higher. Anilinonaphthalene sulfonates (ANS) are essentially nonfluorescent in water, only becoming appreciably fluorescent when bound to membranes.


BisANS induces LLPS of the TDP43 LCD a Chemical structure of

Because ATP and ATP analogs partially displace complexed bis-ANS it has also been possible to study interactions of S-1 and nucleotides by using the displacement effect. Approximate values of the parameters of these various interactions have been measured. Some possible applications of bis-ANS have been explored.


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Depending on context, bis-ANS can both induce LLPS de novo as well as prevent formation of homotypic liquid droplets. Our study also reveals the mechanisms by which bis-ANS and related.


Threedimensional structure of bisANS. For clarity only polar

Bis-ANS is a fluorescent probe for nonpolar cavities in proteins. Used to detect A β fibre (K d = ~80 nM); exhibits distinct fluorescent profiles for fibres and oligomers. Fluoresces in hydrophobic environments, negligible fluorescence in water solutions. Excitation\emission λ ~ 355\520 nm at pH 7.4. Also modulates liquid-liquid phase.


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Despite emergence of bis-ANS as a major fluorescence probe of proteins structure, conformational and spectroscopic properties of protein/bis-ANS complexes remains largely unexplored. We have shown that fluorescence polarization of both ANS and bis-ANS is excitation wavelength dependent and this is a property of all protein-ANS/bis-ANS complexes.


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Bis-ANS is a fluorescent probe for nonpolar cavities in proteins. Used to detect A β fibre (K d = ~80 nM); exhibits distinct fluorescent profiles for fibres and oligomers. Fluoresces in hydrophobic environments, negligible fluorescence in water solutions. Excitation\emission λ ~ 355\520 nm at pH 7.4.


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9619517 DOI: 10.1080/07391102.1998.10508216 Abstract Despite emergence of bis-ANS as a major fluorescence probe of proteins structure, conformational and spectroscopic properties of protein/bis-ANS complexes remains largely unexplored.


Titration of bisANS binding. Binding of bisANS to G protein (A and B

5,5'-Bis [8- (phenylamino)-1-naphthalenesulfonate] (bis-ANS), the fluorescent probe which binds to tubulin, inhibits its assembly into microtubules [Horowitz et al. (1984) J. Biol. Chem. 259, 14647-14650].